| Microorganism name |
Komagatella phaffi (Pichia pastoris)
|
| Target proteins |
Soy leghemoglobin (Shao et al., 2022)
|
| Wild-type or GMO |
GMO (Shao et al., 2022)
|
| Production mode (intracellular/extracellular) |
Extracellular (Shao et al., 2022)
|
| Protein yield (g/L or g/g?) |
3.5 g/L (Shao et al., 2022)
|
| Temperature used in study |
30°C (Shao et al., 2022)
|
| pH used in study |
pH 6.5 (Shao et al., 2022)
|
| C & N source |
Glycerol, (NH4)2SO4 (Shao et al., 2022)
|
| Regulatory status in Europe |
Production of leghemoglobin is issued as safe for consumption by EFSA, but is not allowed for the use food yet (Casacuberta et al., 2024)
|
| Regulatory status in other parts of the world |
|
| Companies |
Impossible foods
|
| Publications/references |
-
Casacuberta, J., Barro, F., Braeuning, A., Cubas, P., De Maagd, R., Epstein, M. M., Frenzel, T., Gallois, J., Koning, F., Messéan, A., Moreno, F. J., Nogué, F., Savoini, G., Schulman, A. H., Tebbe, C., Veromann, E., Gennaro, A., Gonzalez, A. B. G., Ruiz, J. Á. G., . . . Schoonjans, R. (2024). Assessment of soy leghemoglobin produced from genetically modified Komagataella phaffii, under Regulation (EC) No 1829/2003 (application EFSA‐GMO‐NL‐2019‐162). EFSA Journal, 22(11). https://doi.org/10.2903/j.efsa.2024.9060
-
Shao, Y., Xue, C., Liu, W., Zuo, S., Wei, P., Huang, L., Lian, J., & Xu, Z. (2022). High-level secretory production of leghemoglobin in Pichia pastoris through enhanced globin expression and heme biosynthesis. Bioresource Technology, 363, 127884. https://doi.org/10.1016/j.biortech.2022.127884
-
Spohner, S. C., Müller, H., Quitmann, H., & Czermak, P. (2015). Expression of enzymes for the usage in food and feed industry with Pichia pastoris. Journal of Biotechnology, 202, 118–134. https://doi.org/10.1016/j.jbiotec.2015.01.027
-
Lv, W., & Cai, M. (2025). Advancing Recombinant Protein Expression in Komagataella phaffii: Opportunities and Challenges. FEMS Yeast Research. https://doi.org/10.1093/femsyr/foaf010
-
Pan, Y., Yang, J., Wu, J., Yang, L., & Fang, H. (2022). Current advances of Pichia pastoris as cell factories for production of recombinant proteins. Frontiers in Microbiology, 13. https://doi.org/10.3389/fmicb.2022.1059777
-
Barone, G. D., Emmerstorfer-Augustin, A., Biundo, A., Pisano, I., Coccetti, P., Mapelli, V., & Camattari, A. (2023). Industrial Production of Proteins with Pichia pastoris—Komagataella phaffii. Biomolecules, 13(3), 441. https://doi.org/10.3390/biom13030441
-
Ergün, B. G., Laçın, K., Çaloğlu, B., & Binay, B. (2022). Second generation Pichia pastoris strain and bioprocess designs. Biotechnology for Biofuels and Bioproducts, 15(1). https://doi.org/10.1186/s13068-022-02234-7
|